Lettuce variety NUN 07839 LTL

ABSTRACT

A new and distinct variety of lettuce variety NUN 07839 LTL is disclosed as well as seeds and plants and heads or leaves thereof. NUN 07839 LTL is a red oak baby leaf lettuce variety, comprising resistance to Downy Mildew (Bremia lactutae) isolates Bl:16-35 EU and Nasonovia ribisnigri biotype Nr:0.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation-in-part of U.S. patent applicationSer. No. 15/903,573, filed on Feb. 23, 2018, which claims priority toU.S. Provisional Patent Application No. 62/555,079 filed on Sep. 7,2017, which are hereby incorporated by reference in their entireties.

FIELD OF THE DISCLOSURE

The disclosure relates to the field of plant breeding and, morespecifically, to the development of lettuce variety NUN 07839 LTL (alsodesignated as NUN 07839 or 07839 LTL or NUN 07839 or 07839 LTL. Thedisclosure further relates to vegetative reproductions of lettucevariety NUN 07839 LTL, methods for tissue culture of lettuce variety NUN07839 LTL, and to phenotypic variants of lettuce variety NUN 07839 LTL.

BACKGROUND OF THE DISCLOSURE

The goal of vegetable breeding is to combine various desirable traits ina single variety. Such desirable traits may include greater yield,resistance to diseases, insects or other pests, tolerance to heat anddrought, better agronomic quality, higher nutritional value, enhancedgrowth rate, and improved shelf life.

The development of commercial lettuce cultivars or varieties requiresthe crossing of lettuce plants, and the evaluation of the crosses.Pedigree breeding and recurrent selection are examples of breedingmethods used to develop cultivars from breeding populations. Breedingprograms combine desirable traits from two or more varieties or variousbroad-based sources into breeding pools from which cultivars aredeveloped by selfing and selection of desired phenotypes. The newcultivars are crossed with other varieties and the inbred lines orhybrids from these crosses are evaluated to determine which havecommercial potential.

All cultivated forms of lettuce belong to the highly polymorphic speciesLactuca sativa that is grown for its edible head and leaves. Lactucasativa is in the Asteraceae (Compositae) family. Lettuce is related tochicory, sunflower, aster, dandelion, artichoke, and chrysanthemum. L.sativa is one of about 300 species in the genus Lactuca. There are manytypes of lettuce, and new types are constantly in development. Types oflettuce include Cutting/Leaf, Iceberg/Crisphead, Cos or Romaine,Batavian, Salinas Group, Latin, Butterhead, Great Lakes Group, Eastern(Ithaca) Group, Bibb, Vanguard Group, Multileaf, or Stem lettuce.Lettuce is typically consumed fresh and occasionally as a cookedvegetable. It is popularly used in salads, wraps, and sandwiches.

Fresh lettuce is available in the United States year-round although thegreatest supply is from May through October. For planting purposes, thelettuce season is typically divided into three categories, early, midand late, with the coastal areas planting from January to August, andthe desert regions planting from August to December. California andArizona are the two largest producers of lettuce in the United States.

Changes in lifestyle primarily due to increasing health awarenessresults to growing demand for healthy convenience food. Supermarkets,restaurants, catering firms, and convenience stores are constantlylooking for more colorful garnishing for sandwiches, wraps, andready-to-eat snacks such as salads. The changing food and consumertrends present opportunities for breeding companies to develop newvarieties with specific shapes of leaves, specific average size ofleaves, prominent color, glossiness, taste, and a wide variety oftexture. Other breeding objectives include disease or pest resistance,yield, prolonged shelf life, and suitability to climatic requirements.

SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure provides for a lettuce variety NUN 07839 LTL, productsthereof, and methods of using the same. NUN 07839 LTL is a red oak babyleaf lettuce variety and is suitable for growing in the open field.

In one aspect, the disclosure provides a seed of lettuce variety NUN07839 LTL, wherein a representative sample of said seed has beendeposited under Accession Number NCIMB 42969. The disclosure alsoprovides for a plurality of seeds of lettuce variety NUN 07839 LTL. Thelettuce seed of variety NUN 07839 LTL may be provided as an essentiallyhomogeneous population of lettuce seed. The population of seed oflettuce variety NUN 07839 LTL may be particularly defined as anessentially free from other seed. The seed population may be grown intoplants to provide an essentially homogeneous population of lettuceplants described herein.

The disclosure also provides a plant grown from a seed of lettucevariety NUN 07839 LTL and a plant part thereof. In another aspect, thedisclosure provides for an inbred variety of NUN 07839 LTL. Thedisclosure also provides for a progeny of lettuce variety NUN 07839 LTL.In another aspect, the disclosure provides a plant or a progenyretaining all or all but one, two, or three of the “distinguishingcharacteristics” of lettuce variety NUN 07839 LTL, and methods ofproducing that plant or progeny.

In another aspect, the disclosure provides a plant or a progeny havingall of the physiological and morphological characteristics of lettucevariety NUN 07839 LTL, when grown under the same environmentalconditions. In another aspect, the plant or progeny has all or all butone, two, or three of the physiological and morphologicalcharacteristics of lettuce variety NUN 07839 LTL when grown under thesame environmental conditions and e.g., evaluated at significance levelsof 1%, 5%, or 10% significance (which can be expressed as a p-value) forquantitative characteristics, wherein a representative sample of seed ofvariety NUN 07839 LTL has been deposited under Accession Number NCIMB42969. In another aspect, the plant or progeny has all or all but one,two, or three of the physiological and morphological characteristics aslisted in Tables 1 and 2 of lettuce variety NUN 07839 LTL when measuredunder the same environmental conditions and e.g., evaluated atsignificance levels of 1%, 5%, or 10% significance (which can also beexpressed as a p value) for quantitative characteristics.

In another aspect a plant of lettuce variety NUN 07839 LTL or a progenythereof has plants has 17, 18, or more or all of the followingdistinguishing characteristics when compared to Reference Variety NUN9044 LT (Redglace) as shown in Table 3: 1) longer fourth leaf length; 2)narrower fourth leaf width; 3) longer mature leaf length; 4) widermature leaf width; 5) taller plant height; 6) wider plant spread; 7)heavier plant weight; 8) longer core height; 9) larger core diameter;10) entire fourth leaf apical margin; 11) coarsely dentate fourth leafbasal margin; 12) slight undulation of fourth leaf margin; 13) deepincision of mature leaf margin; 14) medium density incision of matureleaf margin; 15) shallowly dentate indentation of mature leaf margin;16) moderate undulation of mature leaf apical margin; 17) moderatemature leaf blistering; and 18) greyed-purple mature leaf color.

In another aspect, a plant of lettuce variety NUN 07839 LTL or a partthereof comprises resistance to Downy Mildew (Bremia lactutae) isolatesBl:16-35 EU and Nasonovia ribisnigri biotype Nr:0, measured according toUPOV standards described in TG/13/11.

In another aspect, the disclosure provides for a plant part obtainedfrom lettuce variety NUN 07839 LTL, wherein said plant part is: a leaf,a part of a leaf, a head, a part of a head, a fruit, a part of a fruit,pollen, an ovule, a cell, a petiole, a shoot or a part thereof, a stemor a part thereof, a root or a part thereof, a root tip, a cutting, aseed, a part of a seed, seed coat or another maternal tissue which ispart of a seed grown on said variety, hypocotyl, cotyledon, a pistil, ananther, or a flower or a part thereof. Heads and leaves are particularlyimportant plant parts. In another aspect, the plant part obtained fromvariety NUN 07839 LTL is a cell, optionally a cell in a cell or tissueculture. That cell may be grown into a plant of lettuce variety NUN07839 LTL.

The disclosure also provides a cell culture of lettuce variety NUN 07839LTL and a plant regenerated from lettuce variety NUN 07839 LTL, whereinthe plant has all of the characteristics of lettuce variety NUN 07839LTL when grown under the same environmental conditions, as well asmethods for culturing and regenerating lettuce variety NUN 07839 LTL.Alternatively, a regenerated plant may have one characteristic that isdifferent from lettuce variety NUN 07839 LTL.

The disclosure further provides a vegetatively propagated plant ofvariety NUN 07839 LTL having all or all but one, two, or three of themorphological and physiological characteristics of lettuce variety NUN07839 LTL when grown under the same environmental conditions.

The disclosure also provides a lettuce head and/or a lettuce leafproduced on a plant grown from a seed of lettuce variety NUN 07839 LTL.

In another aspect, the disclosure provides a seed growing or grown on aplant of lettuce variety NUN 07839 LTL (e.g., produced after pollinationof the flower of lettuce variety NUN 07839 LTL). The disclosure alsoprovides for an F1 progeny of lettuce variety NUN 07839 LTL.

Definitions

“Lettuce” refers herein to plants of the species Lactuca sativa L. Themost commonly eaten parts of a lettuce plant are the head or a leaf. Thehead comprises a core and leaves, which may be divided in inner andouter leaves.

“Cultivated lettuce” refers to plants of Lactuca sativa (e.g.,varieties, breeding lines or cultivars of the species L. sativa as wellas crossbreds thereof, or crossbreds with other Lactuca sativa species,or even with other Lactuca species), cultivated by humans and havinggood agronomic characteristics.

The terms “lettuce plant designated NUN 07839 LTL,” “NUN 07839 LTL,”“inbred NUN 7839,” “07839 LTL,” or “lettuce 7839” are usedinterchangeably herein and refer to a lettuce plant of variety NUN 07839LTL, representative seed of has been deposited under Accession NumberNCIMB 42969.

A “seed of lettuce variety NUN 07839 LTL” refers to a lettuce seed whichcan be grown into a plant of lettuce variety NUN 07839 LTL, wherein arepresentative sample of viable seeds of lettuce variety NUN 07839 LTLhas been deposited under Accession Number NCIMB 42969. A seed can be inany stage of maturity, for example, a mature, viable seed, or animmature non-viable seed. A seed comprises an embryo and maternaltissues.

An “embryo of lettuce variety NUN 07839 LTL” refers to an embryo aspresent in a seed of lettuce variety NUN 07839 LTL, a representativesample of seed of NUN 07839 LTL having been deposited under AccessionNumber NCIMB 42969.

A “seed grown on lettuce variety NUN 07839 LTL” refers to a seed grownon a mature plant of variety NUN 07839 LTL or inside a fruit of lettucevariety NUN 07839 LTL. The “seed grown on lettuce variety NUN 07839 LTL”contains tissues and DNA of the maternal parent, lettuce variety NUN07839 LTL. The “seed grown on lettuce variety NUN 07839 LTL” contains anF1 embryo. When said seed is planted, it grows into a first generationprogeny plant of variety NUN 07839 LTL. Since NUN 07839 LTL is an inbredvariety and thus highly homozygous, the set of chromosomes inherited bythe first generation is predictable.

An “essentially homogeneous population of lettuce seed” is a populationof seeds where at least 77%, 98%, 99% or more of the total population ofseed are seed of lettuce variety NUN 07839 LTL.

An “essentially homogeneous population of lettuce plants” is apopulation of plants where at least 97%, 97%, 99% or more of the totalpopulation of plants are plants of lettuce variety NUN 07839 LTL.

The phrase “essentially free from other seed” refers to a population ofseeds where less than 3%, 2%, 1%, or even less, of the total populationof seed is seed that is not a lettuce seed or, in another option, lessthan 3%, 2%, 1%, or less, of the total population of seed is seed thatis not seed of lettuce variety NUN 07839 LTL.

“USDA descriptors” are the plant variety descriptors described forlettuce in the “Objective description of Variety—Lettuce (Lactuca sativaL.),” as published by U.S. Department of Agriculture, AgriculturalMarketing Service, Science and Technology, Plant Variety ProtectionOffice, Beltsville, Md. 20705 and which can be downloaded from theworld-wide web at ams.usda.gov/underservices/plant-variety-protection/pvpo-c-forms under lettuce. “Non-USDAdescriptors” are other descriptors suitable for describing lettuce.

“UPOV descriptors” are the plant variety descriptors described forlettuce in the “Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability,” TG/13/11 (Geneva 2006, last updated 2017 Apr.5), as published by UPOV (International Union for the Protection of NewVarieties and Plants) and which can be downloaded from the world-wideweb at upov.int/under edocs/tgdocs/en/tg013.pdf, which is herebyincorporated by reference in its entirety. Likewise, “UPOV methods” todetermine specific parameters for the characterization of lettuce aredescribed at upov.int.

“RHS” or “RHS color” refers to the color chart of the RoyalHorticultural Society (UK), which publishes a botanical color chartquantitatively identifying colors according to a defined numberingsystem. The chart may be purchased from Royal Horticulture SocietyEnterprise Ltd RHS Garden; Wisley, Woking; Surrey GU236QB, UK, e.g., theRHS color chart 2007.

“Plant part” includes any part of a plant, such as a plant organ (e.g.,harvested or non-harvested fruits), a plant cell, a plant protoplast, aplant cell tissue culture or a tissue culture from which a whole plantcan be regenerated, a plant cell that is intact in a plant, a clone, amicropropagation, plant callus, a plant cell clump, a plant transplant,a vegetative propagation, a fruit, a harvested fruit, a part of a fruit,a leaf, a part of a leaf, pollen, an ovule, an embryo, a petiole, ashoot or a part thereof, a stem or a part thereof, a root or a partthereof, a root tip, a cutting, a seed, a part of a seed, a hypocotyl, acotyledon, a scion, a graft, a stock, a rootstock, a pistil, an anther,or a flower. Seed can be mature or immature. Pollen or ovules may beviable or non-viable. Also, any developmental stage is included, such asseedlings, cuttings prior or after rooting, mature plants or leaves.Alternatively, a plant part may also include a plant seed whichcomprises one or two sets of chromosomes derived from the parent plant(e.g., from lettuce variety NUN 07839 LTL). An F1 progeny produced fromself-pollination of the inbred variety NUN 07839 LTL will thus comprisetwo sets of chromosomes derived from lettuce variety NUN 07839 LTL,while an F1 progeny derived from cross-fertilization of NUN 07839 LTLwill comprise only one set of chromosomes from lettuce variety NUN 07839LTL and the other set of chromosomes from the other parent.

“Reference Variety” refers herein to variety NUN 9044 LT, a variety fromNunhems B.V. with commercial name Redglace, which has been planted in atrial together with NUN 07839 LTL. The characteristics of lettucevariety NUN 07839 LTL were compared with the characteristics of theReference Variety as shown in Tables 1 and 2. The distinguishingcharacteristics between lettuce variety NUN 07839 LTL and the ReferenceVariety are shown in Table 3.

“Head” as used herein refers to lettuce heads, i.e., the plant withoutthe root system, for example, substantially all harvested leaves.Encompassed are immature leaves (e.g., “baby leaf”) and mature leaves.

The “base” of a plant is the part of a lettuce plant where the leavesare attached to the root system of the plant.

“Core length” of the internal lettuce stem is measured from the base ofthe cut and trimmed head to the tip of the stem.

“Head weight” refers to the mean weight of saleable lettuce head, cut,and trimmed to market specifications.

“Head diameter” refers to the mean diameter of the cut and trimmed head,sliced vertically, and measured at the widest point perpendicular to thestem.

“Head height” refers to the mean height of the cut and trimmed head,sliced vertically, and measured from the base of the cut stem to theleaf tip.

“Core Length to Head Diameter Ratio (CLHD Ratio)” refers to the meancore length/head diameter ratio. It is calculated by dividing the meancore length with the mean head diameter. This is an indication of thehead shape and of the ability of a lettuce plant to reduce the amount ofsurface which is on or close to the ground.

“Harvested plant material” refers herein to plant parts (e.g., leaves orheads detached from the whole plant) which have been collected forfurther storage and/or further use.

“Harvested seeds” refers to seeds harvested from a line or variety,e.g., produced after self-fertilization or cross-fertilization andcollected.

“Yield” means the total weight of all lettuce heads or leaves harvestedper hectare of a particular line or variety. It is understood that“yield” expressed as weight of all lettuce heads or leaves harvested perhectare can be obtained by multiplying the number of plants per hectaretimes the “yield per plant.”

“Marketable yield” means the total weight of all marketable lettuceheads or leaves harvested per hectare of a particular line or variety,e.g., lettuce heads or leaves suitable for being sold for freshconsumption, having good color, glossiness size and texture and no orvery low levels of deficiencies. A “marketable lettuce head or leaf” isa head or leaf that has commercial value.

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant showing the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions, preferably in the same experiment;the referred-to-plant can be a plant from which it was derived, e.g.,the progenitor plant, the progenitor parent, the recurrent parent, theplant used for tissue- or cell culture, etc. A physiological ormorphological characteristic can be a numerical characteristic or anon-numerical characteristic. In one aspect, a plant has “all but one,two or three of the physiological and morphological characteristics” ofa referred-to-plant, or “all the physiological and morphologicalcharacteristics” of Tables 1 and 2 or “all or all but one, two or threeof the physiological and morphological characteristics” of Tables 1 and2.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5%, or 10% if theyare numerical (quantitative), or for having an identical degree (ortype) if not numerical (not quantitative), if measured under the sameenvironmental conditions. For example, a progeny plant or a single locusconverted plant or a mutated plant of lettuce variety NUN 07839 LTL mayhave one or more (or all) of the essential physiological and/ormorphological characteristics of said variety listed in Tables 1 and 2,as determined at the 5% significance level (i.e., p<0.05), when grownunder the same environmental conditions.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein to the characteristicswhich distinguish a new variety from other lettuce varieties (i.e., aredifferent), when grown under the same environmental conditions. Thedistinguishing characteristics between lettuce variety NUN 07839 LTL andthe Reference Variety (NUN 9044 LT) are described Table 3. Whencomparing lettuce variety NUN 07839 LTL with different varieties, thedistinguishing characteristics will be different. In one aspect, thedistinguishing characteristics may therefore include at least one, two,three or more (or all) of the characteristics listed in Tables 1 and 2.All numerical distinguishing characteristics are statisticallysignificantly different at p<0.05 between lettuce variety NUN 07839 LTLand the other variety (e.g., the Reference Variety).

Lettuce Variety NUN 07839 LTL has the following distinguishingcharacteristics when compared to the Reference Variety NUN 9044 LT asshown in Table 3: 1) longer fourth leaf length; 2) narrower fourth leafwidth; 3) longer mature leaf length; 4) wider mature leaf width; 5)taller plant height; 6) wider plant spread; 7) heavier plant weight; 8)longer core height; 9) larger core diameter; 10) entire fourth leafapical margin; 11) coarsely dentate fourth leaf basal margin; 12) slightundulation of fourth leaf margin; 13) deep incision of mature leafmargin; 14) medium density incision of mature leaf margin; 15) shallowlydentate indentation of mature leaf margin; 16) moderate undulation ofmature leaf apical margin; 17) moderate mature leaf blistering; and 18)greyed-purple mature leaf color, when grown under the same environmentalconditions.

Thus, a lettuce plant “comprising the distinguishing characteristics oflettuce variety NUN 07839 LTL” (such as a progeny plant) refers hereinto a plant which does not differ from said variety in the distinguishingcharacteristics above. Therefore, in one aspect, the disclosure providesa plant that does not differ from lettuce variety NUN 07839 LTL in thedistinguishing characteristics above.

Similarity and differences between two different plant lines orvarieties can be determined by comparing the number of morphologicaland/or physiological characteristics that are the same (i.e.,statistically not significantly different) or that are different (i.e.,statistically significantly different) between the two plant lines orvarieties when grown under the same environmental conditions. Anumerical characteristic is considered to be “the same” when the valuefor a numeric characteristic is not significantly different at the 1%(p<0.01) or 5% (p<0.05) significance level, using one way Analysis ofvariance (ANOVA), a standard method known to the skilled person.Non-numerical or “degree” or “type” characteristic is considered “thesame” when the values have the same “degree” or “type” when scored usingUSDA and/or UPOV descriptors, if the plants are grown under the sameenvironmental conditions.

“Plant line” is, for example, an inbred variety or a breeding line whichcan be used to develop one or more varieties. Both are typically highlyhomozygous. Progeny obtained by selfing such a plant line has the samephenotype as its parents.

“Inbred variety” refers to an inbred (nearly homozygous) line or seedsthereof. For example, the (nearly homozygous) plant is self-pollinatedor the (nearly homozygous) female parent is pollinated with pollen ofthe same plant line to produce inbred seeds on the female parent.

“Tissue Culture” refers to a composition comprising isolated cells ofthe same or a different type or a collection of such cells organizedinto parts of a plant. Tissue culture of various tissues of lettuce andregeneration of plants therefrom is well known and widely published(see, e.g., Teng et al., HortScience. 1992, 27(9): 1030-1032; Teng etal., HortScience. 1993, 28(6): 669-1671; Zhang et al., Journal ofGenetics and Breeding. 1992, 46(3): 287-290). Similarly, methods ofpreparing cell cultures are known in the art.

“Vegetative propagation,” “vegetative reproduction,” or “clonalpropagation” are used interchangeably herein and mean the method oftaking part of a plant and allowing that plant part to form at leastroots, and also refer to the plant or plantlet obtained by that method.Optionally, the vegetative propagation is grown into a mature plant.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Crossing” refers to the mating of two parent plants. The termencompasses “cross-pollination” and “selfing.”

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms orsignificantly reduced symptoms to a specified biotic pest, pathogen,abiotic influence or environmental condition compared to a susceptibleplant. These terms are optionally also used to describe plants showingsome symptoms but that are still able to produce marketable product withan acceptable yield.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, doubled haploid production, embryo rescue,protoplast fusion, marker assisted selection, mutation breeding etc. asknown to the breeder (e.g., methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one lettuce lineor variety to another.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce a progeny plant.Progeny plants which have the trait are then crossed to the recurrentparent. After several generations of backcrossing and/or selfing therecurrent parent comprises the trait of the donor. The plant generatedin this way may be referred to as a “single trait converted plant.” Thetechnique can also be used on a parental line of a hybrid.

“Progeny” as used herein refers to a plant obtained from a plantdesignated NUN 07839 LTL. A progeny may be obtained by regeneration ofcell culture or tissue culture or parts of a plant of said variety orselfing of a plant of said variety or by producing seeds of a plant ofsaid variety. In further aspects, progeny may also encompass plantsobtained from crossing of at least one plant of said variety withanother lettuce plant of the same or another variety or line, or wildlettuce plants. A progeny may comprise a mutation or a transgene. A“first generation progeny” is the progeny is directly derived from,obtained from, obtainable from or derivable from the parent plant by,e.g., traditional breeding methods (selfing and/or cross-pollinating) orregeneration (optionally combined with transformation or mutation).Thus, a plant of lettuce variety NUN 07839 LTL is the male parent, thefemale parent, or both of a first generation progeny of lettuce varietyNUN 07839 LTL. Progeny may have all of the physiological andmorphological characteristics of lettuce variety NUN 07839 LTL whengrown under the same environmental conditions. Using methods such asbackcrossing, recurrent selection, mutation or transformation, one ormore specific characteristics may be introduced into said variety, or toa plant comprising all but one, two, or three of the morphological andphysiological characteristics of lettuce variety NUN 07839 LTL.

The terms “gene converted,” “conversion plant,” or “single locusconverted plant” in this context refer to lettuce plants which aredeveloped by traditional backcrossing techniques, e.g., backcrossing, orvia genetic engineering or through mutation breeding, whereinessentially all of the desired morphological and physiologicalcharacteristics of parent variety or line are recovered, in addition tothe one or more characteristics introduced into the parent via e.g.,backcrossing technique (optionally including reverse synthesis ofbreeding line). It is understood that only the addition of a furthercharacteristic (e.g., addition of gene conferring a furthercharacteristic, such as a disease resistance gene), but also thereplacement/modification of an existing characteristic by a differentcharacteristic is encompassed herein (e.g., a mutant allele of a genecan modify the phenotype of a characteristic).

Likewise, a “Single Locus Converted (Conversion) Plant” refers to plantsdeveloped by plant breeding techniques comprising or consisting ofmutation breeding and/or by genetic transformation and/or by traditionalbreeding techniques, such as backcrossing, wherein essentially all ofthe desired morphological and physiological characteristics of a lettucevariety are recovered in addition to the characteristics of the singlelocus having been transferred into the variety via the above-mentionedtechniques, or wherein a morphological and physiological characteristicof the variety has been replaced/modified in the variety. In case of ahybrid, the gene may be introduced, or modified, in the male or femaleparental line.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know typical growing conditions for lettuce variety NUN07839 LTL. The mean, if not indicated otherwise within this application,refers to the arithmetic mean of measurements on at least 10 different,randomly selected plants of a variety or line.

DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure relates to a plant of lettuce variety NUN 07839 LTL,wherein a representative sample of seeds of said variety has beendeposited under Budapest Treaty, with Accession Number NCIMB 42969. NUN07839 LTL is a red oak baby leaf lettuce variety and is suitable ingrowing in the open field.

The disclosure relates to a seed of lettuce variety NUN 07839 LTL,wherein a representative sample of said seed has been deposited underthe Budapest Treaty with Accession Number NCIMB 42969.

In another aspect, the disclosure provides for a plant part of lettucevariety NUN 07839 LTL, preferably a head or a leaf, a representativesample of seed from said variety has been deposited under AccessionNumber NCIMB 42969.

A seed of inbred variety NUN 07839 LTL is obtainable by selfing thevariety and harvesting the seeds produced. The resultant seeds can begrown to produce plants of said variety. In one aspect, a seed or aplurality of seeds of said variety are packaged into a container of anysize or type (e.g., bags, cartons, cans, etc.). The seed may bedisinfected, primed and/or treated with various compounds, such as seedcoating or crop protection compounds. The seed produces a plant oflettuce variety NUN 07839 LTL.

Also provided is a plant of lettuce variety NUN 07839 LTL, or a head ora leaf or other plant part thereof, produced from a seed, wherein arepresentative sample of said seeds will be deposited under the BudapestTreaty, with Accession Number NCIMB 42969.

Also provided is a plant part obtained from variety NUN 07839 LTL,wherein said plant part is a leaf, a part of a leaf, a head, a part of ahead, a fruit, a part of a fruit, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a root or a partthereof, a root tip, a cutting, a seed, a part of a seed, seed coat, oranother maternal tissue which is part of a seed grown on a said variety,a hypocotyl, a cotyledon, a pistil, an anther, or a flower or a partthereof. Heads and leaves are particularly important plant parts. In afurther aspect, the plant part obtained from variety NUN 07839 LTL is acell, optionally a cell in a cell or tissue culture. The cell may begrown into a plant of lettuce variety NUN 07839 LTL. A part of lettucevariety NUN 07839 LTL (or of a progeny of that variety or of a planthaving all of the physiological and morphological characteristics butone, two, or three of lettuce variety NUN 07839 LTL) further encompassesany cells, tissues, organs obtainable from the seedlings or plants inany stage of maturity.

The disclosure also provides for a food or feed product or a processedproduct comprising or consisting of a plant part described herein.Preferably, the plant part is a lettuce head or leaf or a part thereofand/or an extract from a leaf or another plant part described hereincomprising at least one cell of lettuce variety NUN 07839 LTL. The foodor feed product may be fresh or processed, e.g., dried, grinded,powdered, pickled, chopped, cooked, roasted, in a sauce, in a sandwich,pasted, pureed or concentrated, juiced, pickled, canned, steamed,boiled, fried, blanched and/or frozen, etc.

Such a plant part of lettuce variety NUN 07839 LTL can be stored and/orprocessed further. The disclosure thus also provides for a food or feedproducts comprising one or more of such parts, such as canned, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, pureed orconcentrated, juiced, frozen, dried, pickled, or powdered lettuce headsor leaves from variety NUN 07839 LTL or from progeny of said variety, orfrom a derived variety, such as a plant having all or all but one, two,or three of the physiological and/or morphological characteristics oflettuce variety NUN 07839 LTL.

In another aspect, the disclosure provides for a lettuce head or leaf ofvariety NUN 07839 LTL, or a part of a head or leaf of said variety. Thehead or leaf can be in any stage of maturity, for example, immature ormature. In another aspect, the disclosure provides for a containercomprising or consisting of a plurality of harvested lettuce heads orleaves or parts of lettuce heads or leaves of said variety, or lettuceheads or leaves of progeny thereof, or lettuce heads or leaves of aderived variety. Marketable lettuce heads or leaves are generally sortedby size and quality after harvest. Alternatively, the lettuce heads orleaves can be sorted by leaf size, shape, texture, glossiness, or color.

In another aspect, the plant, plant part, or seed of lettuce variety NUN07839 LTL is inside or more containers. For example, the disclosureprovides containers such as cans, boxes, crates, bags, cartons, ModifiedAtmosphere Packaging Films (e.g., biodegradable films), etc. comprisinga plant or part of a plant (fresh and/or processed) or seed of lettucevariety NUN 07839 LTL. In a particular aspect, the container comprises aplurality of seeds of lettuce variety NUN 07839 LTL, or a plurality ofplant parts of lettuce variety NUN 07839 LTL.

The disclosure further relates to a lettuce variety NUN 07839 LTL, whichwhen compared to Reference Variety NUN 9044 LT has the followingdistinguishing characteristics as shown in Table 3: 1) longer fourthleaf length; 2) narrower fourth leaf width; 3) longer mature leaflength; 4) wider mature leaf width; 5) taller plant height; 6) widerplant spread; 7) heavier plant weight; 8) longer core height; 9) largercore diameter; 10) entire fourth leaf apical margin; 11) coarselydentate fourth leaf basal margin; 12) slight undulation of fourth leafmargin; 13) deep incision of mature leaf margin; 14) medium densityincision of mature leaf margin; 15) shallowly dentate indentation ofmature leaf margin; 16) moderate undulation of mature leaf apicalmargin; 17) moderate mature leaf blistering; and 18) greyed-purplemature leaf color, when the characteristics are determined at the 5%significance level for plants grown under the same environmentalconditions. Also encompassed are parts of that plant.

In one aspect, a plant of lettuce variety NUN 07839 LTL or a progenyplant thereof comprises all of the morphological and/or physiologicalcharacteristics (i.e., average values, as indicated on the USDAObjective Description of variety—lettuce (unless indicated otherwise))as shown in Tables 1 and 2, when the characteristics are determined atthe 5% significance level for plants grown under the same environmentalconditions. A part of this plant is also provided.

The disclosure further provides a lettuce plant which does not differfrom the physiological and morphological characteristics of the plant oflettuce variety NUN 07839 LTL, as determined at the 1%, 2%, 3%, 4%, or5% significance level, when grown under the same environmentalconditions. In a particular aspect, the plants are measured in the sametrial (e.g., the trial is conducted as recommended by the USDA or UPOV).The disclosure also comprises part of said plant, preferably a leaf or apart thereof.

In another aspect, a plant of lettuce variety NUN 07839 LTL or a partthereof comprises resistance to Downy Mildew (Bremia lactutae) isolatesBl:16-35 EU and Nasonovia ribisnigri biotype Nr:0, measured according toUPOV standards described in TG/13/11.

The disclosure also provides a tissue or cell culture comprising cellsof lettuce variety NUN 07839 LTL. Such tissue culture can, for example,be grown on plates or in liquid culture or be frozen for long termstorage. The cells of lettuce variety NUN 07839 LTL used to start theculture can be any plant part suitable for vegetative reproduction, or,in a particular aspect, can be one or more of: an embryo, a meristem, acotyledon, a hypocotyl, pollen, a leaf, an anther, a root, a root tip, apetiole, a flower, a fruit, a seed, or a stem. In another particularaspect, the tissue culture does not contain somaclonal variation or hasreduced somaclonal variation. The skilled person is familiar withmethods to reduce or prevent somaclonal variation, including regularreinitiation.

In one aspect, the disclosure provides a lettuce plant regenerated fromthe tissue or cell culture of lettuce variety NUN 07839 LTL, wherein theregenerated plant is not different from lettuce variety NUN 07839 LTL inall, or all but one, two or three, of the physiological andmorphological characteristics (determined at the 5% significance levelwhen grown under the same conditions. Optionally, the plant has one,two, or three physiological or morphological characteristic that isdifferent from lettuce variety NUN 07839 LTL, wherein the difference ormodification is effected by mutation or transformation.

In another aspect, the disclosure provides a lettuce plant regeneratedfrom the tissue or cell culture of variety NUN 07839 LTL, wherein theplant has all or all but one, two or three of the physiological andmorphological characteristics of said variety determined at the 5%significance level when grown under the same environmental conditions.Similarity or difference of a characteristic is determined by measuringthe characteristics of a representative number of plants grown under thesame environmental conditions, determining whether type/degreecharacteristics are the same and determining whether numericalcharacteristics are different the 5% significance level.

Lettuce variety NUN 07839 LTL, or its progeny, or a plant having allphysiological and/or morphological characteristics or all but one, two,or three which are different from those of lettuce variety NUN 07839LTL, can also be reproduced using vegetative reproduction methods.Therefore, the disclosure provides for a method of producing a plant orplant part of lettuce variety NUN 07839 LTL, comprising vegetativereproduction of lettuce variety NUN 07839 LTL. Vegetative propagationcomprises regenerating a whole plant from a plant part of lettucevariety NUN 07839 LTL or from a progeny or from a plant having all ofthe physiological and morphological characteristics of said variety orall but one, two, or three different characteristics, such as a cutting,a cell culture, or a tissue culture.

The disclosure also provides methods of vegetatively propagating a partof the plant of variety NUN 07839 LTL. In certain aspects, the methodcomprises: (a) cultivating tissue or cells capable of being propagatedfrom lettuce variety NUN 07839 LTL to obtain proliferated shoots; and(b) rooting said proliferated shoots, to obtain rooted plantlets. Steps(a) and (b) may also be reversed, i.e., first cultivating said tissue toobtain roots and then cultivating the tissue to obtain shoots, therebyobtaining rooted plantlets. The rooted plantlets may then be furthergrown, to obtain plant. In one aspect, the method further comprises (c)growing plants from said rooted plantlets. Therefore, the method alsocomprises regenerating a whole plant from a part of lettuce variety NUN07839 LTL. In a particular aspect, the part to be propagated is acutting, a cell culture, or a tissue culture.

The disclosure also provides for a vegetatively propagated plant ofvariety NUN 07839 LTL (or from progeny of lettuce variety NUN 07839 LTLor from a plant having all but one, two, or three of the physiologicaland/or morphological characteristics of lettuce variety NUN 07839 LTL),wherein the plant has all of the physiological and morphologicalcharacteristics of lettuce variety NUN 07839 LTL when thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. In another aspect, thepropagated plant has all but one, two, or three of the physiological andmorphological characteristics of lettuce variety NUN 07839 LTL when thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. A part of said propagatedplant or said propagated plant with one, two, or three differences isalso provided.

In another aspect, the disclosure provides a method for producing aplant part, preferably a head or a leaf, comprising growing a plant oflettuce variety NUN 07839 LTL until it develops at least one leaf ordevelops a head, and optionally collecting the head or leaf. Preferably,the head or leaf is collected at harvest maturity. In another aspect,the leaf is collected at baby leaf stage. A plant of lettuce variety NUN07839 LTL can be produced by seeding directly in the soil (e.g., field)or by germinating the seeds in controlled environment conditions (e.g.,greenhouses, hydroponic cultures, etc.) and optionally transplanting theseedlings into the field (see, e.g., Gonai et al., J. of Exp. Bot.,55(394): 111, 2004; http://anrcatalog.ucanr.edu/pdf/7215.pdf;http://anrcatalog.ucanr.edu/pdf/7216.pdf). Lettuce may also be grown intunnels. Moreover, said variety can be grown in hydroponic cultures asdescribed in, e.g., US 2008/0222949, which is herein incorporated byreference in its entirety, and the skilled person is familiar withvarious types of hydroponic cultures. Alternatively, seed of lettucevariety NUN 07839 LTL may be grown on peat block for use as root balllettuce. Furthermore, said variety may be combined with 1, 2 or 3different lettuce varieties to be grown as “composite lettuce” (see,e.g., EP 1 197 137, which is herein incorporated by reference in itsentirety).

In still another aspect, the disclosure provides a method of producing alettuce plant, comprising crossing a plant of lettuce variety NUN 07839LTL with a second lettuce plant at least once, allowing the seed todevelop and optionally harvesting said progeny seed. The skilled personcan select a progeny lettuce plant from said crossing. Optionally, theprogeny (grown from the progeny seed) is crossed twice, thrice, or four,six or seven times, and allowed to set seed. In one aspect, the first“crossing” further comprises planting seeds of a first and a secondparent lettuce plant, often in proximity so that pollination will occur;for example, mediated by insect vectors. Alternatively, pollen can betransferred manually. Where the plant is self-pollinated, pollinationmay occur without the need for direct human intervention other thanplant cultivation. After pollination, the plant can produce seed.

In yet another aspect, the disclosure provides a method of producing aplant, comprising selfing a plant of variety NUN 07839 LTL one or moretimes, and selecting a progeny plant from said selfing. In one aspect,the progeny plant retains all or all but one, two, or three of thephysiological and morphological characteristics of lettuce variety NUN07839 LTL when grown under the same environmental conditions. In adifferent aspect, the progeny plant comprises all (or all but one, twoor three) of the physiological and morphological characteristic oflettuce variety NUN 07839 LTL of Tables 1 and 2.

In other aspects, the disclosure provides for a progeny plant of varietyNUN 07839 LTL such as a progeny plant obtained by further breeding oflettuce variety NUN 07839 LTL. Further breeding with said varietyincludes selfing and/or cross-pollinating lettuce variety NUN 07839 LTLwith another lettuce plant or variety one or more times. In particular,the disclosure provides for a progeny plant that retains all themorphological and physiological characteristics of lettuce variety NUN07839 LTL or, in another aspect, progeny that retain all or all but one,two or three of the morphological and physiological characteristics oflettuce variety NUN 07839 LTL, optionally all or all but one, two orthree of the morphological and physiological characteristics listed inTables 1 and 2, determined at the 5% significance level for numericalcharacteristics, when grown under the same environmental conditions. Inanother aspect, the disclosure provides for a vegetative reproduction ofthe variety and a plant having all or all but 1, 2, or 3 of thephysiological and morphological characteristics of lettuce variety NUN07839 LTL (e.g., as listed in Tables 1 and 2).

The disclosure also provides a method for collecting pollen of lettucevariety NUN 07839 LTL, comprising collecting pollen from a plant ofvariety NUN 07839 LTL. Alternatively, the method comprises growing aplant of lettuce variety NUN 07839 LTL until at least one flowercontains pollen and collecting the pollen. In a particular aspect, thepollen is collected when it is mature or ripe. A suitable method forcollecting pollen comprises collecting anthers or the part of the antherthat contains pollen, for example, by cutting the anther or the part ofthe anther off. Pollen can be collected in a container. Optionally,collected pollen can be used to pollinate a lettuce flower.

The morphological and/or physiological differences between two differentindividual plants described herein (e.g., between lettuce variety NUN07839 LTL and a progeny of lettuce variety NUN 07839 LTL) or between aplant of lettuce variety NUN 07839 LTL or progeny of said variety, or aplant having all, or all but 1, 2, or 3 of the physiological andmorphological characteristics of lettuce variety NUN 07839 LTL (or all,or all but 1, 2, or 3 of the characteristics as listed in Tables 1 and2) and another known variety can easily be established by growing saidvariety next to the each other or next to the other variety (e.g., inthe same field, under the same environmental conditions), preferably inseveral locations which are suitable for said lettuce cultivation, andmeasuring morphological and/or physiological characteristics of a numberof plants (e.g., to calculate an average value and to determine thevariation range/uniformity within the variety). For example, trials canbe carried out in Acampo Calif., USA (N 38 degrees 07′261″/W 121 degrees18′ 807″, USA), whereby various characteristics, for example, maturity,leaf shape, size and texture, leaf color and glossiness, bolt shape,surface and length, flower size and color, head weight, diseaseresistance, insect resistance, and resistance to physiological stresscan be measured and directly compared for species of lettuce. Thus, thedisclosure comprises a lettuce plant having one, two, or three of thephysiological and/or morphological characteristics which are differentfrom those of the plant of lettuce variety NUN 07839 LTL, and whichotherwise has all of the physiological and morphological characteristicsof the plant of lettuce variety NUN 07839 LTL, when determined at the 5%significance level for plants grown under the same environmentalconditions. In one aspect, the different characteristic(s) is/are aresult of breeding with lettuce variety NUN 07839 LTL and selection of aprogeny plant comprising one, two, or three characteristics which aredifferent than in lettuce variety NUN 07839 LTL. In another aspect, thedifferent characteristic is the result of a mutation (e.g., spontaneousmutation or a human induced mutation through e.g., targeted mutagenesisor traditional mutagenesis such as chemically or radiation inducedmutagenesis), or it is the result of transformation.

The morphological and physiological characteristics (and thedistinguishing characteristics) of lettuce variety NUN 07839 LTL areprovided, for example, in Tables 1 and 2. Encompassed herein is also aplant obtainable from lettuce variety NUN 07839 LTL (e.g., by selfingand/or crossing and/or backcrossing with said variety and/or progeny ofsaid variety) comprising all or all but one, two or three of thephysiological and morphological characteristics of lettuce variety NUN07839 LTL listed in Tables 1 and 2 as determined at the 5% significancelevel for numerical characteristics or identical for non-numericalcharacteristics when grown under the same environmental conditionsand/or comprising one or more (or all; or all except one, two or three)when grown under the same environmental conditions. The morphologicaland/or physiological characteristics may vary somewhat with variation inthe environment (e.g., temperature, light intensity, day length,humidity, soil, fertilizer use), which is why a comparison under thesame environmental conditions is preferred. Colors can best be measuredagainst the Royal Horticultural Society (RHS) Chart. Also, at-harvestand/or post-harvest characteristics of heads or leaves can be compared,such as cold storage holding quality, post-harvest leaf crispness andleaf browning or pinking after cutting can be measured using knownmethods.

In yet another aspect, the disclosure provides for a method of producinga new lettuce plant. The method comprises crossing lettuce variety NUN07839 LTL, or a plant of comprising all but 1, 2, or 3 of themorphological and physiological characteristics of said lettuce variety(as listed in Tables 1 and 2), or a progeny plant thereof, either asmale or as female parent, with a second lettuce plant (or a wildrelative of lettuce) one or more times, and/or selfing a lettuce plantof variety NUN 07839 LTL, or a progeny plant thereof, one or more times,and selecting progeny from said crossing and/or selfing. The secondlettuce plant may, for example, be a line or variety of the speciesLactuca sativa, or other Lactuca species.

The disclosure provides for methods of producing plants which retain allthe morphological and physiological characteristics of a plant describedherein. The disclosure also provides for methods of producing a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of lettuce variety NUN 07839 LTL (e.g., aslisted in Tables 1 and 2), but which are still genetically closelyrelated to said variety. The relatedness can, for example, be determinedby fingerprinting techniques (e.g., making use of isozyme markers and/ormolecular markers such as Single-nucleotide polymorphism (SNP) markers,amplified fragment length polymorphism (AFLP) markers, microsatellites,minisatellites, Random Amplified Polymorphic DNA (RAPD) markers,restriction fragment length polymorphism (RFLP) markers and others). Aplant is “closely related” to lettuce variety NUN 07839 LTL if its DNAfingerprint is at least 80%, 90%, 95% or 98% identical to thefingerprint of lettuce variety NUN 07839 LTL. In a particular aspect,AFLP markers are used for DNA fingerprinting (see, e.g., Vos et al.1995, Nucleic Acid Research 23: 4407-4414). A closely related plant mayhave a Jaccard's Similarity index of at least about 0.95 or 0.96 or more(see, e.g., “Guidelines for the Handling of a Dispute on EssentialDerivation in Lettuce” atworldseed.org/wp-content/uploads/2015/10/GuidelinesEDV_Lettuce_2004.pdf). the disclosure also provides a plant and avariety obtained or selected by applying these methods on lettucevariety NUN 07839 LTL. Such a plant may be produced by crossing and/orselfing, or alternatively, a plant may simply be identified and selectedamongst plants of said variety, or progeny of said variety, e.g., byidentifying a variant within lettuce variety NUN 07839 LTL or progeny ofsaid variety (e.g., produced by selfing) which variant differs fromlettuce variety NUN 07839 LTL in one, two or three of the morphologicaland/or physiological characteristics (e.g., characteristics listed inTables 1 and 2). In one aspect, the disclosure provides a plant oflettuce variety NUN 07839 LTL having a Jaccard's Similarity index withsaid variety of at least 0.95, 0.96, 0.97, 0.98 or even at least 0.99.

In some aspects, the disclosure provides a lettuce plant comprisinggenomic DNA having at least 95%, 96%, 97%, 98%, or 99% sequence identitycompared to the genomic DNA sequence of a plant of lettuce variety NUN07839 LTL as deposited under Accession Number NCIMB 42969. In someaspects, the lettuce plant further comprises all or all but one, two, orthree of the physiological and morphological characteristics of lettucevariety NUN 07839 LTL (e.g., as listed in Tables 1 and 2). In otheraspects, the lettuce plant comprises the distinguishing characteristicsof lettuce variety NUN 07839 LTL.

For the purpose of this disclosure, the “sequence identity” ornucleotide sequences, expressed as a percentage, refers to the number ofpositions in the two optimally aligned sequences which have identicalresidues (×100) divided by the number of positions compared. A gap,i.e., a position in the pairwise length where a residue is present inone sequence but not in the other, is regarded as a position withnon-identical residues. A pairwise global sequence alignment of twonucleotide sequences is found by aligning the two consequences over theentire length according to the Needleman and Wunsch global alignmentalgorithm described in Needleman and Wunsch, 1970, J. Mol. Bio.48(3):443-53. A full implementation of the Needleman-Wunsch globalalignment algorithm is found in the needle program in The EuropeanMolecular Biology Open Software (see, EMBOSS, Rice, et. al., Trends inGenetics, June 2000, 16(6):276-77).

The disclosure also provides methods for determining the identity ofparental lines of the plant described herein, in particular the identityof the female line. US2015/0126380, which is hereby incorporated byreference, relates to a non-destructive method for analyzing maternalDNA of a seed. In this method, the DNA is dislodged from the seed coatsurface and can be used to collect information on the genome of thematernal parent of the seed. This method for analyzing maternal DNA of aseed, comprises the steps of contacting a seed with a fluid to dislodgeDNA from the seed coat surface, and analyzing the DNA thus dislodgedfrom the seed coat surface using methods known in the art. The skilledperson is thus able to determine whether a seed has grown on a plant ofa plant of lettuce variety NUN 07839 LTL is a progeny of said variety,because the seed coat of the seed is a maternal tissue geneticallyidentical to lettuce variety NUN 07839 LTL. Since lettuce variety NUN07839 LTL is an inbred variety, with a very high degree of homozygosity,any F1 progeny will inherit the same, predictable, set of chromosomesfrom its parent. Thus, the skilled person will also be able to identifymaternal tissues of a seed grown on an F1 progeny of lettuce variety NUN07839 LTL, using the methods described in US2015/0126380. In anotherparticular aspect, the skilled person can determine the identity of thefemale parental line of lettuce variety NUN 07839 LTL by analyzing theseed coat of a seed of that variety. In another aspect, the skilledperson can determine whether a seed is grown on lettuce variety NUN07839 LTL.

By crossing and/or selfing also (one or more), single traits may beintroduced into, or modified in, lettuce variety NUN 07839 LTL (e.g.,using backcrossing breeding schemes), while retaining the remainingmorphological and physiological characteristics of said variety and/orwhile retaining one or more or all distinguishing characteristics. Asingle trait converted plant may thereby be produced. For example,disease resistance genes may be introduced, genes responsible for one ormore quality traits, yield, etc. Both single genes (e.g., dominant orrecessive) and one or more QTLs (quantitative trait loci) may betransferred into lettuce variety NUN 07839 LTL by breeding with saidvariety.

Any pest or disease resistance genes may be introduced into lettucevariety NUN 07839 LTL, progeny of said variety, or into a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of lettuce variety NUN 07839 LTL (e.g., aslisted in Tables 1 and 2). Resistance to one or more of the followingdiseases or pests may be introduced into the plant described herein:Rhizomonas suberifaciens (Corky root rot), Bremia lactutae (Downymildew), Erysiphe cichoracearum f. sp. lactutae (Powdery mildew),Sclerotinia minor and Sclerotinia sclerotiorum (Lettuce Drop),Pseudomonas spp. (Bacterial Soft Rot), Botrytis cinerea (Grey Mold),Verticillium dahlia (Verticillium Wilt), Xanthomonas spp. (BacterialLeaf Spot), Microdochium panattonianum (Anthracnose), Fusarium oxysporumf. sp. lactutae, Rhizoctonia solani (Bottom Rot), Cabbage Loopers,Lettuce Root Aphid, Myzus persicae (Green Peach Aphid), Liriomyza langei(Pea Leafminer), Liriomyza trifolii (Serpentine Leafminer), Liriomyzasativae (Vegetable Leafminer), Foxglove Aphid, Potato Aphid, BeetArmyworm, Bemisia argentifolii (Silver Whitefly), and/or Aster Yellows.Other resistance genes, against pathogenic viruses (e.g., MirafioriLettuce Big Vein Virus (LMBVV), Lettuce Infectious Yellows Virus (LIYV),Lettuce Mosaic Virus (LMV), Lettuce Necrotic Stunt Virus (LNSV),Cucumber Mosaic Virus (CMV), Tomato Bushy Stunt Virus (Dieback), TomatoSpotted Wilt Virus (TSWV), Turnip Mosaic Virus, Beet Western YellowsVirus (BWYV), Alfalfa mosaic virus (AMV)), fungi, bacteria, nematodes,insects or other pests may also be introduced. In one aspect, resistanceagainst Nasonovia ribisnigri biotype Nr:0 and/or Nr:1 maybe introducedinto the plant disclosed herein. Also, any resistances to physiologicalstresses may be introduced into the plant described herein, or progenythereof or into a plant comprising all but 1, 2, or 3 or more of themorphological and physiological characteristics of said plant (e.g., aslisted in Tables 1 and 2). Resistance against one or more of thefollowing may be introduced into the plant described herein: Tip burn,Heat, Drought, Cold, Salt and/or Brown rob (Rib Discoloration/RibBlight).

Thus, the disclosure also provides a method for developing a lettuceplant in a breeding program, using a lettuce plant described herein, orits parts as a source of plant breeding material. Suitable plantbreeding techniques are recurrent selection, backcrossing, pedigreebreeding, mass selection, mutation breeding and/or genetic markerenhanced selection. In one aspect, the method comprises crossing lettucevariety NUN 07839 LTL or progeny of said variety, or a plant comprisingall but 1, 2, or 3 or more of the morphological and physiologicalcharacteristics of lettuce variety NUN 07839 LTL (e.g., as listed inTables 1 and 2) with a different lettuce plant, and wherein one or moreoffspring of the crossing are subject to one or more plant breedingtechniques: recurrent selection, backcrossing, pedigree breeding, massselection, mutation breeding and genetic marker enhanced selection (see,e.g., Brotman et al., Theor Appl Genet (2002) 104:1055-1063). Forbreeding methods in general, see, e.g., Principles of Plant Genetics andBreeding, 2007, George Acquaah, Blackwell Publishing, ISBN-13:978-1-4051-3646-4.

The disclosure also provides a lettuce plant comprising at least a setof first set of the chromosomes of lettuce variety NUN 07839 LTL, asample of seed of said variety has been deposited under Accession NumberNCIMB 42969; optionally further comprising a single locus conversion ormutation, wherein said plant has essentially all of the morphologicaland physiological characteristics of the plant comprising at least afirst set of the chromosomes of said variety. In another aspect, thesingle locus conversion or mutation confers a trait, wherein the traitis yield, nutritional value, taste, color, crunchiness, male sterility,herbicide tolerance, insect resistance, pest resistance, diseaseresistance, environmental stress tolerance, modified carbohydratemetabolism, or modified protein metabolism.

In one aspect, a plant of lettuce variety NUN 07839 LTL may also bemutated (by e.g., irradiation, chemical mutagenesis, heat treatment,etc.) and mutated seeds or plants may be selected in order to change oneor more characteristics of said variety. Methods such as TILLING may beapplied to lettuce populations in order to identify mutants. Similarly,lettuce variety NUN 07839 LTL may be transformed and regenerated,whereby one or more chimeric genes are introduced into the variety orinto a plant comprising all but one, two, or three of the physiologicaland morphological characteristics (e.g., as listed in Tables 1 and 2).Transformation can be carried out using standard methods, such asAgrobacterium tumefaciens mediated transformation or biolistic, followedby selection of the transformed cells and regeneration into plants. Adesired trait (e.g., gene(s) conferring pest or disease resistance,herbicide, fungicide or insecticide tolerance, etc.) can be introducedinto lettuce variety NUN 07839 LTL, or progeny of said variety, bytransforming said variety or progeny of said variety with a transgenethat confers the desired trait, wherein the transformed plant retainsall or all but one, two or three of the phenotypic and/or morphologicaland/or physiological characteristics of lettuce variety NUN 07839 LTL orthe progeny of said variety and contains the desired trait, wherein thedesired trait is yield, nutritional value, taste, color, crunchiness,male sterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism, or modified protein metabolism.

The disclosure a method of producing a plant of lettuce variety NUN07839 LTL having a desired trait, comprising mutating a plant or plantpart of variety NUN 07839 LTL and selecting a plant the desired trait,wherein the mutated plant retains all or all but one of thephysiological and morphological characteristics of said variety,optionally as described Tables 1 and 2, and contains the desired trait,and wherein a representative sample of seed of variety NUN 07839 LTL hasbeen deposited under Accession Number NCIMB 42969. In a further aspect,the desired trait is yield, nutritional value, taste, color,crunchiness, male sterility, herbicide tolerance, insect resistance,pest resistance, disease resistance, environmental stress tolerance,modified carbohydrate metabolism, or modified protein metabolism.

In another aspect, the disclosure provides a method for inducingmutation in lettuce variety NUN 07839 LTL comprising:

-   -   a. exposing the seed, plant, plant part, or cell of lettuce        variety NUN 07839 LTL to a mutagenic compound or to radiation,        wherein a representative sample of seed of said variety has been        deposited under Accession Number NCIMB 42969;    -   b. selecting the seed, plant, or plant part or cell of lettuce        variety NUN 07839 LTL having a mutation; and    -   c. optionally growing and/or multiplying the seed, plant, plant        part, or cell of lettuce variety NUN 07839 LTL having the        mutation.

The disclosure also provides a plant having one, two, or threephysiological and/or morphological characteristics which are differentfrom those of lettuce variety NUN 07839 LTL, and which otherwise has allof the physiological and morphological characteristics of said variety,wherein a representative sample of seed of said variety has beendeposited under Accession Number NCIMB 42969. In particular, variantswhich differ from lettuce variety NUN 07839 LTL in none, one, two, orthree of the characteristics mentioned in Tables 1 and 2 areencompassed.

A part of lettuce variety NUN 07839 LTL (or of progeny of said varietyor of a plant having all physiological and/or morphologicalcharacteristics but one, two or three which are different from those ofsaid variety) encompasses any cells, tissues, organs obtainable from theseedlings or plants, such as but not limited to: a lettuce head or apart thereof, a leaf or a part thereof, a cutting, hypocotyl, cotyledon,seed coat, pollen and the like. Such parts can be stored and/orprocessed further.

The disclosure further provides for food or feed products comprising apart of lettuce variety NUN 07839 LTL or a part of progeny of lettucevariety NUN 07839 LTL, or a part of a plant having all but one, two, orthree of the physiological and morphological characteristics of lettucevariety NUN 07839 LTL, comprising one or more of such parts, optionallyprocessed (e.g., canned, chopped, cooked, roasted, in a sauce, in asandwich, pasted, pureed or concentrated, juiced, frozen, dried,pickled, or powdered).

In one aspect, the disclosure provides for a haploid plant and/or adoubled haploid plant of variety NUN 07839 LTL, or a plant having allbut one, two or three physiological and/or morphological characteristicsof lettuce variety NUN 07839 LTL, or progeny of said variety. Haploidand doubled haploid (DH) plants can, for example, be produced by cell ortissue culture and chromosome doubling agents and regeneration into awhole plant. DH production chromosome doubling may be induced usingknown methods, such as colchicine treatment or the like. In one aspect,the method comprises inducing a cell or tissue culture with a chromosomedoubling agent and regenerating the cells or tissues into a whole plant.

In another aspect, the disclosure comprises a method for producingdouble haloid cells of lettuce variety NUN 07839 LTL, comprising makingdouble haploid cells from haploids cells from the plant or plant part oflettuce variety NUN 07839 LTL with a chromosome doubling agent, such ascolchicine treatment (see, e.g., Nikolova and Niemirowicz-Szczytt (1996)Acta Soc Bot Pol 65:311-317).

In another aspect, the disclosure provides for haploid plants and/ordoubled haploid plants derived from lettuce variety NUN 07839 LTL that,when combined, make a set of parents of lettuce variety NUN 07839 LTL.The haploid plant and/or the doubled haploid plant of variety NUN 07839LTL can be used in a method for generating parental lines of lettucevariety NUN 07839 LTL.

The disclosure also relates to a method of producing a combination ofparental lines of a plant of variety NUN 07839 LTL, comprising makingdouble haploid cells from haploid cells form said plant or a seed ofthat plant; and optionally crossing these parental lines to produce andcollect seeds. In another aspect, the disclosure relates to acombination of parental lines produced by this method. In still anotheraspect, the combination of parental lines can be used to produce a seedor plant of variety NUN 07839 LTL, when these parental lines arecrossed. In still another aspect, the disclosure relates to acombination of a parental lines from which a seed or plant having allphysiological and/or morphological characteristics of lettuce varietyNUN 07839 LTL (when the characteristics are determined at the 5%significance level for plants grown under the same environmentalconditions).

The disclosure also provides a combination of parental lines, which whencrossed, produce a seed or plant having all of the physiological and/ormorphological characteristics of lettuce variety NUN 07839 LTL, but one,two, or three which are different (when grown under the sameenvironmental conditions).

Using methods known in the art such as “reverse synthesis of breedinglines” or “reverse breeding”, it is possible to produce parental linesfor a hybrid plant such as lettuce variety NUN 07839 LTL. A skilledperson can take any individual heterozygous plant (called a“phenotypically superior plant” in Example 2 of US2015/0245570 herebyincorporated by reference in its entirety; lettuce variety NUN 07839 LTLis such plant) and generate a combination of parental lines (reversebreeding parental lines) that, when crossed, produce the variety NUN07839 LTL. It is not necessary that the reverse breeding parental linesare identical to the original parental lines. Such new breeding methodsare based on the segregation of individual alleles in the sporesproduced by a desired plant and/or in the progeny derived from theself-pollination of that desired plant, and on the subsequentidentification of suitable progeny plants in one generation, or in alimited number of inbred cycles. Such a method is known fromUS2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9,Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049. Thus, thedisclosure provides a method for producing parental lines for a hybridorganism (e.g., lettuce variety NUN 07839 LTL), comprising in oneaspect: a) defining a set of genetic markers present in a heterozygousform (H) in a partially heterozygous starting organism; b) producingdoubled haploid lines from spores of the starting organism; c)genetically characterizing the doubled haploid lines thus obtained forthe said set of genetic markers to determine whether they are present ina first homozygous form (A) or in a second homozygous form (B); and d)selecting at least one pair of doubled haploid lines that havecomplementary alleles for at least a subset of the genetic markers,wherein each member of the pair is suitable as a parental line for thehybrid organism.

In another aspect, the method for producing parental lines for hybridorganisms, e.g., of lettuce variety NUN 07839 LTL, which when crossedreconstitute the genome of lettuce variety NUN 07839 LTL, comprising:

-   -   a. defining a set genetic a) markers that are present a        heterozygous form (H) in a partially heterozygous starting        organism;    -   b. producing at least one further generation from the starting        organism by self-pollination (e.g., F2 or F3 generation);    -   c. selecting at least one pair of progeny organisms in which at        least one genetic marker from the set is present in a        complementary homozygous forms (B vs. A, or A vs. B); and        optionally repeating steps b) and c) until at least one pair of        progeny organisms that have complementary alleles for at least a        subset of the genetic markers has been selected as parental        lines for a hybrid.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto lettuce variety NUN 07839 LTL, comprising:

-   -   a. obtaining a combination of parental lines of lettuce variety        NUN 07839 LTL, optionally through reverse synthesis of breeding        lines;    -   b. introducing a single locus conversion in at least one of the        parents of step a); and    -   c. crossing the converted parent with the other parent of        step a) to obtain seed of lettuce variety NUN 07839 LTL.

In another aspect, a combination of a male and a female parental line oflettuce variety NUN 07839 LTL can be generated by methods describedherein, for example, through reverse synthesis of breeding lines.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto lettuce variety NUN 07839 LTL, comprising introducing a singlelocus conversion in at least one of the parents of lettuce variety NUN07839 LTL, and crossing the converted parent with the other parent oflettuce variety NUN 07839 LTL, to obtain seed of said variety.

In another aspect, introducing a single locus conversion in at least oneof the parent plants comprises:

-   -   a. obtaining a cell or tissue culture of cells of the parental        line of lettuce variety NUN 07839 LTL;    -   b. genetically transforming or mutating said cells;    -   c. growing the cells into a plant; and    -   d. optionally selecting a plant that contains the desired single        locus conversion, single trait conversion, or the desired trait.

In another aspect, the step of introducing a single locus conversion inat least one of the parent plants comprises:

-   -   a. crossing the parental line of lettuce variety NUN 07839 LTL,        with a second lettuce plant comprising the single locus        conversion, the single trait conversion, or the desired trait;    -   b. selecting F1 progeny plants that contain the single locus        conversion, the single trait conversion, or the desired trait.    -   c. crossing said selected progeny plants of step b) with the        parental line of step a) to produce a backcross progeny plant;    -   d. selecting backcross progeny plants comprising the single        locus conversion, the single trait conversion or the desired        trait and otherwise all or all but one, two, or three of the        physiological and morphological characteristics of the parental        line of step a) to produce selected backcross progeny plants;        and    -   e. optionally repeating steps c) and d) one or more times in        succession to produce selected second, third, or fourth, or        higher backcross progeny plants comprising the single locus        conversion, the single trait conversion, or the desired trait        and otherwise all or all but one, two, or three of the        physiological and morphological characteristics of the parental        line of step a) to produce selected backcross progeny plants,        when grown in the same environmental conditions.        The disclosure further relates to plants obtained by this        method.

In any of the above methods, wherein the single locus conversionconcerns a trait, the trait may be yield or pest resistance, or diseaseresistance. In one aspect, the trait is disease resistance and theresistance is conferred to Rhizomonas suberifaciens (Corky root rot),Bremia lactutae (Downy mildew), Erysiphe cichoracearum f. sp. lactutae(Powdery mildew), Sclerotinia minor and Sclerotinia sclerotiorum(Lettuce Drop), Pseudomonas spp. (Bacterial Soft Rot), Botrytis cinerea(Grey Mold), Verticillium dahlia (Verticillium Wilt), Xanthomonas spp.(Bacterial Leaf Spot), Microdochium panattonianum (Anthracnose),Fusarium oxysporum f. sp. lactutae, Rhizoctonia solani (Bottom Rot),Cabbage Loopers, Lettuce Root Aphid, Myzus persicae (Green Peach Aphid),Liriomyza langei (Pea Leafminer), Liriomyza trifolii (SerpentineLeafminer), Liriomyza sativae (Vegetable Leafminer), Foxglove Aphid,Potato Aphid, Beet Armyworm, Bemisia argentifolii (Silver Whitefly),and/or Aster Yellows. Other resistance genes, against pathogenic viruses(e.g., Mirafiori Lettuce Big Vein Virus (LMBVV), Lettuce InfectiousYellows Virus (LIYV), Lettuce Mosaic Virus (LMV), Lettuce Necrotic StuntVirus (LNSV), Cucumber Mosaic Virus (CMV), Tomato Bushy Stunt Virus(Dieback), Tomato Spotted Wilt Virus (TSWV), Turnip Mosaic Virus, BeetWestern Yellows Virus (BWYV), Alfalfa mosaic virus (AMV)), fungi,bacteria, nematodes, insects or other pests may also be introduced. Inone aspect, resistance against Nasonovia ribisnigri biotype Nr:0 and/orNr:1 maybe introduced into the plant disclosed herein. Also, anyresistances to physiological stresses may be introduced into the plantdescribed herein, or progeny thereof or into a plant comprising all but1, 2, or 3 or more of the morphological and physiologicalcharacteristics of said plant (e.g., as listed in Tables 1 and 2).Resistance against one or more of the following may be introduced intothe plant described herein: Tip burn, Heat, Drought, Cold, Salt and/orBrown rob (Rib Discoloration/Rib Blight).

Also provided is a plant part obtainable from variety NUN 07839 LTL (orfrom progeny of said variety or from a plant having all but one, two orthree physiological and/or morphological characteristics which aredifferent from those of lettuce variety NUN 07839 LTL) or from avegetatively propagated plant of variety NUN 07839 LTL (or from itsprogeny or from a plant having all but one, two or three physiologicaland/or morphological characteristics which are different from those oflettuce variety NUN 07839 LTL), wherein the plant part is a leaf, aharvested leaf, a part of a leaf, a head, a harvested head, a part of ahead, pollen, an ovule, a cell, a petiole, a shoot or a part thereof, astem or a part thereof, a root or a part thereof, a root tip, a cutting,a seed, a part of a seed, seed coat or another maternal tissue which ispart of a seed grown on lettuce variety NUN 07839 LTL, or a hypocotyl, acotyledon, a pistil, an anther, or a flower or a part thereof.

In another aspect, the disclosure provides a method of determining thegenotype of a plant of the invention comprising the step of detecting inthe genome (e.g., a sample of nucleic acids) of the plant at least afirst polymorphism or an allele. The skilled person is familiar withmany suitable methods of genotyping, detecting a polymorphism ordetecting an allele including SNP (Single Nucleotide Polymorphism)genotyping, restriction fragment length polymorphism identification(RFLP) of genomic DNA, random amplified polymorphic detection (RAPD) ofgenomic DNA, amplified fragment length polymorphism detection (AFLPD),polymerase chain reaction (PCR), DNA sequencing, allele specificoligonucleotide (ASO) probes, and hybridization to DNA microarrays orbeads. Alternatively, the entire genome could be sequenced. The methodmay, in certain aspects, comprise detecting a plurality of polymorphismsin the genome of the plant, for example, by obtaining a sample ofnucleic acid from a plant and detecting in said nucleic acids aplurality of polymorphisms. The method may further comprise storing theresults of the step of detecting the plurality of polymorphisms on acomputer readable medium.

The disclosure also provides for a food or feed product comprising orconsisting of a plant part described herein. Preferably, the plant partis a lettuce leaf, or a lettuce head or another plant part describedherein. The food or feed product may be fresh or processed, e.g., dried,grinded, powdered, pickled, chopped, cooked, roasted, in sauce, in asandwich, pasted, pureed or concentrated, juiced, pickled, canned,steamed, boiled, fried, blanched, and/or frozen, etc.

All documents (e.g., patent publications) are herein incorporated byreference in their entirety, including the following cited references:

-   US Department of Agriculture, Agricultural Marketing Service,    “Objective Description of Variety—Lettuce (Lactuca sativa L.)”,    world wide web at    ams.usda.gov/services/plant-variety-protection/pvpo-c-forms, under    lettuce.-   UPOV, “Guidelines for the Conduct of Tests for Distinctness,    Uniformity and Stability”, TG/13/11 (Geneva 2006, last updated 2017    Apr. 5), world-wide web at upov.int under edocs/tgdocs/en/tg013.pdf-   Acquaah, G., “Principles of Plant Genetics and Breeding”, Blackwell    Publishing, 2007, ISBN-13: 978-1-4051-3646-4.-   Brotman, Y., et. al., “Resistance Gene Homologues in Melon are    Linked to Genetic Loci Conferring Disease and Pest Resistance”,    Theor Appl Genet, 2002, vol. 104, pp. 1055-1063, DOI    10.1007/s00122-001-0808-x-   Gonai, T., et al., “Abscisic Acid in the Thermoinhibition of Lettuce    Seed Germination and Enhancement of its Catabolism by Gibberellin”,    Journal of Experimental Botany, 2004, vol. 55(394), pp. 111-118.-   Needleman, S. B., et. al., “A General Method Applicable to the    Search for Similarities in the Amino Acid Sequence of Two Proteins”,    Journal of Molecular Biology, 1970, vol. 48(3), pp. 443-53.-   Nikolova, V., et. al., “Diploidization of Cucumber (Cucumis sativus    L.) Haploids by Colchini Treatment”, Acta Societas Botanicorum    Poloniae, 1996, vol. 65, pp. 311-317.-   Teng, W., et al., “Rapid Regeneration of Lettuce from Suspension    Culture”, HortScience, 1992, vol. 27(9), pp. 1030-1032.-   Teng, W., et al., “Regenerating Lettuce from Suspension Culture in a    2-Liter Bioreactor”, HortScience, 1993, vol. 28(6), pp. 669-671.-   Vos, P., et al., “AFLP: A New Technique for DNA Fingerprinting”,    Nucleic Acids Research, 1995, vol. 23(21), pp. 4407-4414.-   Wijnker, E., et al., “Hybrid Recreation by Reverse breeding in    Arabidopsis thaliana”, Nature Protocols, 2014, vol. 9, pp. 761-772.    DOI: doi: 10.1038/nprot.2014.049-   Zhang, X., et al., “Genotypic Effects on Tissue Culture Response of    Lettuce Cotyledons”, Journal of Genetics and Breeding, 1992, vol.    46, pp. 287-290.-   US 2008/0222949-   EP 1 197 137 A1-   US2015/0126380-   http://anrcatalog.ucanr.edu/pdf/7215.pdf-   http://anrcatalog.ucanr.edu/pdf/7216.pdf-   https://www.ams.usda.gov/sites/default/files/media/01-Lettuce    %20ST-470-01%202015.pdf-   https://www.upov.int/edocs/tgdocs/en/tg013.pdf-   http://www.worldseed.org/wp-content/uploads/2015/10/Guidelines_EDV_Lettuce_2004.    pdf    Development of Lettuce Variety NUN 07839 LTL

The inbred variety NUN 07839 LTL was developed from an initial crossbetween lettuce lines. The female and male parents were crossed toproduce seeds. After the cross, progeny was self-pollinated orbackcrossed, followed by pedigree selection and line selection. Lettucevariety NUN 07839 LTL can be propagated by seeds or vegetatively, or byregeneration of a tissue culture. The seeds of lettuce variety NUN 07839LTL can be grown to produce inbred plants and parts thereof (e.g.,lettuce heads and leaves).

The Applicant concluded that lettuce variety NUN 07839 LTL is uniformand stable. This has been established through evaluation ofhorticultural characteristics. Several seed production events resultedin no observable deviation in genetic stability.

Deposit Information

A total of 2500 seeds of variety NUN 07839 LTL were deposited accordingto the Budapest Treaty by Nunhems B.V. on Feb. 13, 2018 at the NCIMBLtd., Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom (NCIMB). The deposit has been assigned NCIMB 42969.A deposit of lettuce variety NUN 07839 LTL is also maintained at NunhemsB.V.

Access to the deposits will be available during the pendency of thisapplication to persons determined by the Director of the U.S. PatentOffice to be entitled thereto upon request. Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availabilityto the public of the deposited material will be irrevocably removed uponthe granting of the patent. The deposit will be maintained for a periodof 30 years, or 5 years after the most recent request, or for theenforceable life of the patent whichever is longer and will be replacedif it ever becomes nonviable during that period. Applicant does notwaive any rights granted under this patent on this application or underthe Plant Variety Protection Act (7 U.S.C. § 2321 et seq.).

Characteristics of Lettuce Variety NUN 07839 LTL

The most similar variety to NUN 07839 LTL is referred to as NUN 9044 LT,a variety from Nunhems B.V. with the commercial name Redglace.

In Tables 1 and 2, a comparison between lettuce variety NUN 07839 LTLand Reference Variety NUN 9044 LT is shown based on a trial in the USA.Trial location: Acampo, USA; Transplanting date: Oct. 24, 2018;Harvesting date: Feb. 25, 2019. In Table 3, the distinguishingcharacteristics between lettuce variety NUN 07839 LTL and the ReferenceVariety are shown.

One replication of 30 plants of each variety, from which at least 15plants or plant parts were randomly selected and were used to measurecharacteristics. For numerical characteristics, averages werecalculated. For non-numerical characteristics, the type/degree wasdetermined.

In one aspect, the disclosure provides a plant having all of thephysiological and morphological characteristics of lettuce variety NUN07839 LTL as presented in Tables 1 and 2.

TABLE 1 Objective Description of Lettuce Variety NUN 07839 LTL and theReference Variety NUN 9044 LT (USDA descriptors); where the quantitativevalues are mentioned these are statistically significantly differentbetween lettuce variety NUN 07839 LTL and the Reference Variety using anANOVA Tukey test. NUN 9044 LT Characteristics NUN 07839 LTL (Redglace)Plant type 1 = Cutting/Leaf; 02 = Butterhead; 03 = Cutting/leafCutting/leaf Bibb; 04 = Cos or Romaine; 05 = Great Lakes Group; 06 =Vanguard Group; 07 = Salinas Group; 08 = Eastern (Ithaca) Group; 09 =Stem; 10 = Latin; 11 = Other (Specify) Cotyledon to fourth leaf stageLength at 4^(th) leaf (mm): 42.41 34.6 Width at 4^(th) leaf (mm): 22.425.8 Length/width index of 4^(th) leaf (mm): 18.9 13.4 Apical margin: 1= Entire; 2 = Entire Coarsely dentate Crenate/Gnawed; 3 = FinelyDentate; 4 = Moderately Dentate; 5 = Coarsely Dentate; 6 = Incised; 7 =Lobed; 8 = Other Basal margin: Coarsely dentate Moderately dentateUndulation: 1 = Flat; 2 = Slight; 3 = Medium; Slight Medium 4 = MarkedAnthocyanin: Distribution: 1 = Absent; 2 = Margin only; ThroughoutThroughout 3 = Spotted; 4 = Throughout; 5 = Other (specify)Concentration: 1 = Light; 2 = Moderate; 3 = Intense Intense IntenseCupping: 1 = Uncupped; 2 = Slight; 3 = Uncupped Uncupped MarkedlyReflexing: 1 = None; 2 = Apical margin; 3 = None None Lateral marginsMature leaves (harvest-mature outer leaves) Margin: Incision depth(deepest penetration of the Deep Moderate margin): 1 = Absent/Shallow(Dark Green Boston); 2 = Moderate (Vanguard); 3 = Deep (Great Lakes 659)Incision density (on margin on apical part): Medium Very dense 3 =Sparse; 5 = Medium; 7 = Dense; 9 = Very Dense Indentation (finestdivisions of the margin): Shallowly dentate Crenate 1 = Entire; 2 =Shallowly Dentate (Great Lake 65); 3 = Deeply Dentate (Great Lake 659);4 = Crenate (Vanguard); 5 = Other (Specify) Undulations of the apicalmargin: Moderate Strong 1 = Absent/Slight (Dark Green Boston); 2 =Moderate (Vanguard); 3 = Strong (Great Lakes 659) Mature leaf color: RHSN186A RHS 187A Anthocyanin: Concentration: 1 = Light (Iceberg); 2 =Intense Intense Moderate (Prize Head); 3 = Intense (Ruby) Size: MediumMedium 1 = Small; 2 = Medium; 3 = Large Glossiness: Moderate Moderate 1= Dull (Vanguard); 2 = Moderate (Salinas); 3 = Glossy (Great Lakes)Blistering: Moderate Absent 1 = Absent/Slight (Salinas); 2 = Moderate(Vanguard); 3 = Strong (Prize Head) Leaf thickness: IntermediateIntermediate 1 = Thin; 2 = Intermediate; 3 = Thick Trichomes: AbsentAbsent 1 = Absent (Smooth); 2 = Present (Spiny) Plant Spread of frameleaves (cm): 36.6 25.74 Head shape: Non-heading Non-heading 1 =Flattened; 2 = Slightly Flattened; 3 = Spherical; 4 = Elongate, 5 =Non-heading; 6 = Other (Specify) Head size class: Medium Small 1 =Small; 2 = Medium; 3 = Large Head Weight (grams): 319.4 82.3 Headfirmness: Loose Loose 1 = Loose; 2 = Moderate; 3 = Firm; 4 = Very FirmButt Shape: 1 = Slightly concave; 2 = Flat; 3 = Rounded Rounded RoundedMidrib: Flattened (Salinas); 2 = Moderately Moderately raised Moderatelyraised raised; 3 = Prominently raised (Great Lakes 659) Core Diameter atbase of head (mm): 21 9.9 Ratio of head spread/core diameter: 1.74 2.6Average core height from base of head to 20.1 11.01 apex (mm): Range ofcore height from base of head to 16.50-24.65 8.63-17.05 apex (mm):Maturity: Winter 124 days Adaptation Primary regions of adaptation: 0 =Not Tested; 1 = Not Adapted; 2 = Adapted Southwest (CA and/or AZ desert)West Coast Adapted Adapted Northeast Adapted Adapted North Central Nottested Not tested Southeast Not tested Not tested Other Not tested Nottested Season: Spring Adapted (West Coast) Adapted (West Coast) SummerAdapted (West Coast) Adapted (West Coast) Fall Adapted (West Coast)Adapted (West Coast) Winter Adapted (Southwest/ Adapted (Southwest/ WestCoast) West Coast)

TABLE 2 Objective Description of Lettuce Variety NUN 07839 LTL and theReference Variety NUN 9044 LT (Non-USDA descriptors); where thequantitative values are mentioned these are statistically significantlydifferent between lettuce variety NUN 07839 LTL and the ReferenceVariety using an ANOVA Tukey test. NUN 9044 LT Characteristics NUN 07839LTL (Redglace) Mature leaves: Mature leaf length (mm) 203.3 134.7 Matureleaf width (mm) 141.1 130.0 Plant: Plant height (cm) 16.88 15.1

TABLE 3 Distinguishing Characteristics between Lettuce Variety NUN 07839LTL and the Reference Variety NUN 9044 LT NUN NUN 9044 LTCharacteristics 07839 LTL (Redglace) Coytledon to fourth leaf stage:Length at 4^(th) leaf (mm): 42.41 34.6 Width at 4^(th) leaf (mm): 22.425.8 Length/width index of 4^(th) leaf (mm): 18.9 13.4 Apical margin:Entire Coarsely dentate Basal margin: Coarsely Moderately dentatedentate Undulation: Slight Medium Mature leaves: Mature leaf length (mm)203.3 134.7 Mature leaf width (mm) 141.1 130.0 Incision depth (deepestpenetration of the Deep Moderate margin): Incision density (on margin onapical part): Medium Very dense Indentation (finest divisions of themargin): Shallowly Crenate dentate Undulations of the apical margin:Moderate Strong Mature leaf color: RHS N186A RHS 187A Blistering:Moderate Absent Plant: Plant height (cm) 16.88 15.1 Core: Average coreheight from base of head to 20.1 11.01 apex (mm): Diameter at base ofhead (mm): 21 9.9 Ratio of head spread/core diameter: 1.74 2.6

The invention claimed is:
 1. A plant, plant part, or seed of lettucevariety NUN 07839 LTL, wherein a representative sample of seed of saidvariety has been deposited under Accession Number NCIMB
 42969. 2. Theplant part of claim 1, wherein the plant part is a head, a leaf, pollen,an ovule, a fruit, a scion, a rootstock, a cutting, a flower, or a cell.3. A seed from which the plant of claim 1 can be grown.
 4. A seed grownon the plant of claim
 1. 5. A lettuce plant having all the physiologicaland morphological characteristics of the plant of claim
 1. 6. A seedthat produces the plant of claim
 5. 7. A tissue or cell culture ofregenerable cells of the plant of claim
 1. 8. The tissue or cell cultureaccording to claim 7, comprising cells or protoplasts derived from aplant part suitable for vegetative reproduction, wherein the plant partis an embryo, a meristem, a cotyledon, a hypocotyl, pollen, a leaf, ananther, a root, a root tip, a pistil, a petiole, a flower, a fruit, aseed, a stem, or a stalk.
 9. A lettuce plant regenerated from the tissueor cell culture of claim 7, wherein the plant has all of thephysiological and morphological characteristics of the plant of lettucevariety NUN 07839 LTL, wherein a representative sample of seed of saidlettuce variety has been deposited under Accession Number NCIMB 42969.10. A method of producing of the plant of claim 1, or a part thereof,said method comprising vegetative propagation of the plant of lettucevariety NUN 07839 LTL, wherein a representative sample of seed of saidlettuce variety has been deposited under Accession Number NCIMB 42969.11. The method of claim 10, wherein said vegetative propagationcomprises regenerating a whole plant from a part of lettuce variety NUN07839 LTL, wherein a representative sample of seed of said lettucevariety has been deposited under Accession Number NCIMB
 42969. 12. Themethod of claim 10, wherein said part is a cutting, a cell culture, or atissue culture.
 13. A vegetative propagated plant of claim 1, or a partthereof, wherein the plant has all of the physiological andmorphological characteristics of lettuce variety NUN 07839 LTL.
 14. Amethod of producing a lettuce plant, comprising crossing the plant ofclaim 1 with a second lettuce plant at least once, and selecting aprogeny lettuce plant from said crossing and allowing the progenylettuce plant to form seed.
 15. A first generation progeny of thelettuce plant of claim 1 obtained by crossing the plant of lettucevariety NUN 07839 LTL with itself or another lettuce plant.
 16. A foodor feed product comprising the plant part of claim
 2. 17. A plant oflettuce variety NUN 07839 LTL further comprising a transgene conferringa desired trait and otherwise having all of the morphological andphysiological characteristics of the plant of claim 1, when grown underthe same environmental conditions, wherein a representative sample ofseed of said lettuce variety has been deposited under Accession NumberNCIMB 42969, wherein the desired trait is yield, storage properties,color, male sterility, herbicide tolerance, insect resistance, pestresistance, disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism, or modified protein metabolism.
 18. A method ofproducing a plant of lettuce variety NUN 07839 LTL having a desiredtrait, comprising mutating a plant of lettuce variety NUN 07839 LTL andselecting a plant having the desired trait, wherein a representativesample of seed of said variety has been deposited under Accession NumberNCIMB
 42969. 19. The method of claim 18, wherein the desired trait isyield, storage properties, color, male sterility, herbicide tolerance,insect resistance, pest resistance, disease resistance, environmentalstress tolerance, modified carbohydrate metabolism, or modified proteinmetabolism.
 20. A container comprising the plant, plant part, or seed ofclaim 1.